The Algae blooms are of considerable concern in freshwater lakes and reservoirs worldwide. In-situ Chlorophyll a (Chl-a) fluorometers are widely used for rapid assessments of algae biomass. However, accurately converting Chla fluorescence to an equivalent concentration is challenging due to natural variations in the relationship as well as nonphotochemical quenching (NPQ) which occurs commonly in surface waters during daytime. This study is based on water quality data from a freshwater lake from October 2018 to December 2020. Initial analysis of sonde Chl-a fluorescence and laboratory extracted Chl-a concentrations shows that the two data sets exhibit a nonlinear relationship with positive correlation and significant errors. A bias correction method was next developed based on (1) concurrent sonde measurements of other water quality parameters (to account for nonlinearities) and (2) a bias correction approach for nonphotochemical quenching effects in surface waters. The new Chl-a model exhibits much improved accuracy, with a root mean square error (RMSE) less than 0.95 mu g/L. The new method facilitates accurate Chl-a characterization in freshwater lakes and reservoirs based on readily obtainable in-situ fluorescence sonde measurements.

（来源：WATER RESEARCH 卷: 205出版年: 2021, DOI: 10.1016/j.watres.2021.117661）